Fig. 2From: An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig spermComet assay Olive Tail Moment (OTM) after incubation of sperm with different concentrations of Mn2+/Ca2+ (A) or Mg2+/Ca2+ (B) (0 mM, 0.1 mM, 1 mM, 5 mM and 50 mM), in non-permeabilized (−TX) and permeabilized (+TX) samples. (*) indicates significant differences between non-permeabilized (−TX) and permeabilized (+TX) samples (P ≤ 0.05)Back to article page