Table 1 Used primer pairs
Gene | Forward primer | Reverse primer | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|
Nos1 (NM_052799.1) | GCA GAG GCC GTC AAG TTC T | GAG AAT GGT CGC CTT GAC CC | 60 | 72 |
Nos2 (NM_012611.3) | AAA CGC TAC ACT TCC AAC GC | TGC TGA GAG CTT TGT TGA GGT C | 59 | 91 |
Nos3 (NM_021838.2) | GAT CCC CCG GAG AAT GGA GA | TCG GAT TTT GTA ACT CTT GTG CT | 60 | 105 |
Cbs (NM_012522.2) | ATG GTG ACT CTC GGG AAC ATG | AGG TGG ATC GGC TTG AAC TG | 59 | 104 |
Ace2 (NM_001012006.2) | TCA GAG CTG GGA TGC AGA AA | GGC TCA GTC AGC ATG GAG TTT | 60 | 111 |
Mas1 NM_012757.2) | TTC ATA GCC ATC CTC AGC TTC TTG | GTT CTT CCG TAT CTT CAC CAC CAA | 60 | 84 |
Rpl10a (NM_031065.1) | TCC ACC TGG CTG TCA ACT TC | GGC AGC AAC GAG GTT TAT TGG | 60 | 134 |