Fig. 3
The effect of MLN and ZOFE treatment on the integrated pressure responses of the cardiovascular system. The changes in mean blood pressure (∆MAP) were induced by infusion of acetylcholine (1 µg/kg, a), noradrenaline (1 µg/kg, b), NOsynthase inhibitor (L-NAME, 30 mg/kg, c), ACE inhibitor captopril (10 mg/kg, e) and H2S scavenger bismuth(III) subsalicylate (BSC, 0.25 µg/kg, (f). The effect of acetylcholine (1 µg/kg) was also investigated before and after pretreatment with L-NAME (30 mg/kg, (d). SHR – spontaneously hypertensive rats (n = 8), SHR + MLN – spontaneously hypertensive rats treated by MLN-4760 (n = 8), SHR + ZOFE – spontaneously hypertensive rats treated by zofenopril (n = 8), SHR + MLN + ZOFE – spontaneously hypertensive rats treated by MLN-4760 and zofenopril (n = 8). The results are presented as the mean ± S.E.M., and differences between groups were analyzed by two-way ANOVA, *** p < 0.001 vs. SHR, + p < 0.05 vs. SHR + MLN, # p < 0.05, ## p < 0.01 vs. before L-NAME