Fig. 1

Equilibrium concentration of angiotensin peptides (a-d). The effect of MLN and ZOFE treatments on the equilibrium plasma renin activity (e) and activity of soluble RAS enzymes determined from angiotensin product/substrate (pmol/L/pmol/L) ratios in plasma (f,g). PRA – plasma renin activity, ACE-S – soluble angiotensin converting enzyme, ALT-S – total alternative renin angiotensin system activity, SHR – spontaneously hypertensive rats (n = 10), SHR + MLN – spontaneously hypertensive rats treated by MLN-4760 (n = 10), SHR + ZOFE – spontaneously hypertensive rats treated by zofenopril (n = 10), SHR + MLN + ZOFE – spontaneously hypertensive rats treated by MLN-4760 and zofenopril (n = 10). The results are presented as the mean ± S.E.M., and differences between groups were analyzed by two-way ANOVA, * p < 0.05, *** p < 0.001 vs. SHR, ⁺⁺ p < 0.01, ⁺⁺⁺ p < 0.001 vs. SHR + MLN