Fig. 4

Atl-KD in motor neurons increases the colocalization of Rab11 with CSP. A Illustration of the protocol for acquiring STED images from WT and Atl-KD Drosophila NMJ, using UAS constructs Rab 4, Rab 7 and Rab 11-GFP fusion proteins overexpressed in motoneurons with C380 driver. Cartoon created with BioRender.com. B-D) STED microscopy images (maximum intensity Z projection) of control (C380) and Atl-KD NMJ boutons expressing a UAS-Rab4-GFP B, endogenous Rab7-GFP C and UAS-Rab11-GFP D. Scale bar: 2 µm. CSP antibody stain is represented in Magenta. Image intensity and contrast have been increased for visualization purposes. Scale bar: 2 µm. Manders 2 coefficients (GFP signal colocalized with CSP marker, divided by total GFP signal) of Rab4 E, Rab7 F, and Rab11 G. Each scatter dot represents one larva. Mann–Whitney, p-value * < 0,05, n = 5–6 larvae