Fig. 3

Atl-KD in motor neuron increases FM 1–43 unloading. A Illustration showing FM1-43 technique to evaluate SV dynamics on Drosophila larval NMJ, see the text for further details. Cartoon created with BioRender.com. B–G Black and white representative spinning disc images (maximum intensity Z projection) of NMJ boutons of non-fixed preparation loaded with FM1-43 dye of different genotypes. Images after load (B1-3 and F1-3) and unload (C1-3 and G1-3) protocols. Image intensity and contrast have been increased for visualization purposes. Scale bar: 20 µm. D, H quantification of the fluorescence intensity of FM 1–43 normalized to control levels after the loading protocol. E, I quantification of the fluorescence intensity of FM 1–43 normalized to control levels after the unloading protocol. Each data point represents the average of several boutons in one larva. Kruskal–Wallis, p-value * < 0,05, ** < 0.01, n = 6–9 larvae