Fig. 9

MiR-212-5p reduces neuronal apoptosis and attenuates axonal degeneration in PC12 cells following OGD/R. A Cleaved-Caspase 3 immunofluorescence staining (in green). Nuclei were stained with DAPI and are visualised in blue. Scale bar = 50 μm. B, C Nogo-A and NgR gene expression levels in PC12 cells. Gene expression levels were examined using qRT–PCR and normalised to β-actin. D-F Immunofluorescence staining was conducted to reveal the presence of Nogo-A, NgR and β III tubulin in PC12 cells. Nuclei were stained with DAPI and are visualised in blue. Scale bar = 50 μm. The data are presented as the means ± SEM (n = 5 per group). *P < 0.05 and **P < 0.01 compared with the sham group, ^#P < 0.05 and ^##P < 0.01 compared with the MCAO/R group