Fig. 8

Agomir-212-5p suppressed the PLXNA2 and RhoA/ROCK2 in PC12 cells following OGD/R. A Representative photographs of western blots showing PLXNA2, RhoA and ROCK2 levels in each group. β-Actin was used as an internal reference. B Quantitative analysis of the western blot results. C-E Immunofluorescence staining results for the PLXNA2, RhoA and ROCK2 (in green) proteins in neurons in each group. Nuclei were stained with DAPI and are visualised in blue. Scale bar = 50 μm. F-H qRT–PCR was performed to assess the mRNA levels of PLXNA2, RhoA and ROCK2 in each group. The data are presented as the means ± SEM (n = 4–6 per group). *P < 0.05 and **P < 0.01 compared with the sham group, ^#P < 0.05 and ^##P < 0.01 compared with the MCAO/R group