Fig. 6

MiR-212-5p promotes synaptic plasticity and attenuates axon degeneration at 7 days following MCAO/R. A Ultrastructures of the synapses in the ischemic penumbra analysed using TEM. Scale bar = 2 μm. B Schematic of the presynaptic (violet) and postsynaptic (green) structures. C The number of synapses in the ischemic penumbra of the cortex in each group. D Width of the synaptic space (nm). n = 3 per group. E Immunofluorescence staining shows MAP-2 (in green) expression in the ischemic penumbra of the cortex. Nuclei were stained with DAPI and are visualised in blue. Scale bar = 50 μm. F Representative images of western blots for Nogo-A, NgR, and GAP-43. G Quantitative analysis of the western blot results. n = 4–6 per group. H-J Immunofluorescence staining was performed to show the presence of Nogo-A, NgR and β III tubulin in the ischemic penumbra of the cortex. Nuclei were stained with DAPI and are visualised in blue. Scale bar =, 50 μm. The data are presented as the means ± SEM. *P < 0.05 and **P < 0.01 compared with the sham group, ^#P < 0.05 and ^##P < 0.01 compared with the MCAO/R group