Fig. 5

Agomir-212-5p inhibited the PLXNA2 and RhoA/ROCK2 in the ischemic penumbra at 3 days following MCAO/R. A The asterisks denote the observation site. B PLXNA2, RhoA and ROCK2 mRNA expression in the ipsilateral brain was analysed using qRT–PCR. C Representative images of western blots for PLXNA2, RhoA and ROCK2 in each group. β-Actin was detected as an internal reference. D Quantitative analysis of the western blot results. E Immunofluorescence staining for the PLXNA2, RhoA and ROCK2 proteins in neurons from each group. Scale bar = 50 μm. The data are presented as the means ± SEM (n = 4–6 per group). *P < 0.05 and **P < 0.01 compared with the sham group, ^#P < 0.05 and ^##P < 0.01 compared with the MCAO/R group