Fig. 4

Agomir-212-5p reduces the infarct volume and mitigates neuronal apoptosis 7 days after MCAO/R. A Brain sections were stained with TTC to visualise the ischemic lesions at 7 days after MCAO/R. The white area shows the infarct core. B Quantitative analysis of the percentage of the infarct area. C H&E staining. Scale bars = 1000 μm (low magnification) and 50 μm (high magnification). D Nissl staining. Scale bars = 50 μm. E, F Immunofluorescence staining for NeuN showing the survival of neurons in the peri-infarct cortex 7 days after MCAO/R. G, H The numbers of NeuN + viable neurons in the peri-infarct striatum. The data are presented as the means ± SEM (n = 3 per group). *P < 0.05 and **P < 0.01 compared with the sham group, ^#P < 0.05 and ^##P < 0.01 compared with the MCAO/R group