Fig. 5
Top 25 altered metabolic pathways and scatter plots showing the impacted metabolic pathways in 3- and 7-day-induced anoikis-resistant or wild-type NCI-H460 cells. These pathways were identified and annotated by comparisons with two databases: the Small Molecule Pathway Database (A and B) and the Kyoto Encyclopedia of Genes and Genomes (C and D). The top 25 altered metabolic pathways were ranked based on the significance (p) values and the enrichment ratios (A and C). The scatter plots were constructed based on the significance (p) value (color scale) and impact score (plot size) of each pathway (B and D). Some plots that associated with the top ranked pathways and having relatively high p values, enrichment ratios, and impact scores, were determined, including i = phosphatidylethanolamine biosynthesis, ii = phosphatidylcholine biosynthesis, iii = β-alanine metabolism, iv = glutathione metabolism, and v = D-glutamine and D-glutamate metabolism (B and D)