Fig. 3

Prolonged anchorage-free lifetimes of NCI-H460 cells do not significantly influence 2-dimensional collective migration but promote early 3-dimensional individual migration. Either 3- and 7-day-induced anoikis-resistant or wild-type cells were assessed for their capability to migrate collectively for up to 24 h and individually for 3 h by a wound-healing assay (A and B) and a transwell migration method (C), respectively. (A) A set of representative views demonstrating the variation in wound areas measured at 0, 12, and 24 h of the incubation, where scale bars = 100 μm. (B) The change in wound areas was computed and reported as a relative wound area to that detected at time zero. (C) A set of representative views (scale bars = 100 μm) showing cells migrated to the bottom part of the transwell membrane after staining with Hoechst 33342 and the average migrated cell counts, where *** = p < 0.0005. All graphical results represent means ± standard deviations derived from at least triplicate experiments