Fig. 2

Prolonged anchorage-free lifetimes of NCI-H460 cells strengthen their survival and growth as shown by a soft agar colony formation assay. Either 3- and 7-day-induced anoikis-resistant or wild-type cells were assessed for their capability to survive and grow under the anchorage independence conditions. The number (A) and size (B) of colonies formed in the soft agar medium was measured, where ns = not significant, *** = p < 0.0001, and n = number of colonies assessed. (C) A set of representative views demonstrating the variation in density and size of colonies survived and grown in the soft agar medium for 7 days, was randomly selected, where scale bars = 100 μm and 50 μm (for the close-up views). All graphical results represent means ± standard errors of means derived from at least triplicate experiments