Fig. 1
Prolonged anchorage-free lifetimes of NCI-H460 cells enhance their survival, proven by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-based cell viability assay. Either 3- and 7-day-induced anoikis-resistant or wild-type cells were assessed for their capability to survive in low-nutrient anchorage-free conditions and recover their adherent growth ability. (A) The percentage of cell viability was computed and reported as a relative percentage value to that measured at time zero. (B) Regression curves generated from the plots between cell viability percentages and sampling times in A were mathematically interpreted, where Y0 = y-intercept, K = slope, and R2 = coefficient of determination. (C) The absorbance values for cell samples collected at time zero and measured by the MTT-based cell viability assay after allowing cell attachment for up to 27 h, were reported, where ns = not significant and ** = p < 0.01. (D) A set of representative views demonstrating dead cells (stained with both bright blue of Hoechst 33342 and red of propidium iodide) after being without anchorage for 24 h assessed by a dual nuclear staining method, was randomly selected, where scale bars = 5 μm. All graphical results represent means ± standard deviations derived from at least triplicate experiments