Fig. 5

miR-219a negatively regulated NMDAR in normal and ischemic brain slices

(A) fEPSP recording in normal-induced brain slices. Baseline recordings were taken 20 min before LTP induction, and 30 min after LTP induction. Drug delivery (green represents the miR-219a inhibitors and blue the miR-219a mimics. 30 min of induction) (B) fEPSP recording in 2-VO-induced brain slices. Bar graphs show the percentage change in LTP after miR-219a inhibitor treatment. *. Sham vs. miR-219a inhibitor, P<0.05 (left). Bar graphs show the percentage change in LTP with miR-219a mimics. *. Sham vs. miR-219a inhibitor, #. MCAO vs. EA, P<0.05 (right)C. Baseline recordings were taken 20 min before LTP induction, and 30 min after LTP induction. Drug delivery (purple represents the miR-219a inhibitors and red the miR-219a mimics. 30 min of induction). DE. Bar graphs show the percentage change in LTP after miR-219a inhibitors treatment on 2VO rats. *. 2VO vs. miR-219a inhibitor, P<0.05 (left) Bar graphs show the percentage change in LTP after miR-219a mimics treatment on 2VO rats (right). E. Effect of miR-219a on amplitude and area under the curve of NMDAR-mediated eEPSC. F. NMDA-eEPSC recordings in hippocampal CA1 neurons from WT and WT + miR-219a mimics *. WT vsWT + miR-219a mimics, P<0.05.(B, D One-way ANOVA, followed by a Tukey’s post hoc test, F. Two-way ANOVA, followed by a Tukey’s post hoc test. n = 6 sections from 3 rats/group)