Fig. 6
From: Human VDAC pseudogenes: an emerging role for VDAC1P8 pseudogene in acute myeloid leukemia
a-b. Expression of VDAC1 gene and VDAC1P8 pseudogene in HL60 AML cell line. In (a), the expression of VDAC1 gene and VDAC1P8 pseudogene was evaluated in different models of leukemia cell line (HL60, IMS-M2, MOLM-13, AML-3, AML-2) by real-time PCR relative quantification. In (b), the expression of VDAC1 and VDAC1P8 both assessed in HL60 by real-time PCR relative quantification were also measured following 48 h of AzaD treatment. The variation of mRNA in treated samples were expressed relatively to control samples, after normalization with the housekeeping gene GAPDH, by the ΔΔCt method. VDAC1P8 expression is reported as % respect to VDAC1 indicating with a value of 100 the level of VDAC1. Data were statistically analyzed by T-test and a value of P < 0.05 was considered significant.