Fig. 6 

HBOT and rapamycin increase ISCs proliferation independently. A–D Representative immunohistochemistry images for proliferating cells, labeled by BrdU incorporation, in intestinal crypts for each experimental group. 20 days of treatment in each group. Nuclei labeled by hematoxylin counterstaining. Bar = 20 µm. Yellow dotted lines outline the crypt periphery. White arrowheads show BrdU + cells. E Quantification of BrdU + cells for each treatment. The same letters indicate no statistical significance while different letters indicate statistical significance. Permutation test. < 0.05. n = 5 per group