Fig. 3

Loss of the ABCA1 activity reduces the ability of cells to form active invadopodia. A, B Representative confocal microscopy images of scrambled and ABCA1 KO cells (A) or DMSO- and probucol-treated cells (B) seeded on gelatin-fluorescein (green) coverslips and F-actin stained with phalloidin-Alexa Fluor 568 (red). White arrowheads point at gelatin degrading invadopodia and yellow arrowheads non-active invadopodia. Scale bars represent 20 µm. C, D Quantification of cell number degrading gelatin of scrambled and ABCA1 KO cells (C) or DMSO- and probucol-treated cells (D) expressed as % of cells degrading gelatin from the total number of cells analyzed (n = 30 from three independent experiments, unpaired Student t-test). E, F Quantification of cells number forming invadopodia of scrambled and ABCA1 KO cells (E) or DMSO-and probucol-treated cells (F) expressed as % of invadopodia forming cells from the total number of cells analyzed (n = 30 from three independent experiments unpaired Student t-test). Data are mean ± SD. *p ≤ 0.05 and **p ≤ 0.01