Fig. 7

Acupuncture intervention in PCOS rats increases the number of granulocytes. A Experimental strategy for acupuncture intervention; B Number of granulosa cells in the ovaries of PCOS rats after acupuncture intervention; C Primary granulosa cells were extracted and cultured to observe cell number. The number of granulosa cells in the ovaries of PCOS rats were significantly increased after acupuncture intervention (scale bar = 100 μm). A-C, N = 6.These data are expressed as the mean ± standard deviation, ***P < 0.0001 compared to the normal group and ^####P < 0.0001 compared to the sh-NC and PCOS groups. One-way factorial ANOVA and Tukey’s post hoc test were used for statistical analysis. 1: Normal group, 2: PCOS group, 3: PCOS + A group, 4: PCOS + SA group, 5: PCOS + W group