Fig. 3

Silencing MEG3 ameliorates abnormal sex hormone levels and ovarian histopathological changes in PCOS rats. A Experimental strategy for the inhibition of ovarian MEG3 expression by viral injection; B HE staining was used to observe pathological changes in the ovaries of PCOS rats after MEG3 downregulation (scale bar = 200 μm); C Number of mature follicles in the ovarian tissue of PCOS rats after MEG3 downregulation; D Number of corpus luteum structures in the ovarian tissue of PCOS rats after MEG3 downregulation; E Serum secretion of LH in PCOS rats after silencing MEG3; F Serum secretion of AMH in PCOS rats after silencing MEG3; G Serum secretion of FSH in PCOS rats after silencing MEG3;H T secretion in the serum of PCOS rats after MEG3 silencing; I E2 secretion in the serum of PCOS rats after MEG3 silencing. A-I, N = 6. These data are expressed as the mean ± standard deviation, *P < 0.05, **P < 0.01, ***P < 0.0001 compared with the normal group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.0001 compared with the sh-NC group, and statistically analysis using one-way ANOVA and Tukey's post hoc test. e2 stands for oestradiol, AMH stands for anti-Mullerian hormone, T stands for testosterone, FSH stands for follicle-stimulating hormone, and LH stands for luteinizing hormone. 1: Normal group, 2: PCOS group, 3:sh-MEG3 group, 4:sh-NC group