Fig. 2

MEG3 binds to miR-21-3p, a direct target gene of miR-21-3p in PCOS rats. A Putative complementary binding site of miR-21-3p and MEG3; B Fluorophore activity assay verified the targeting relationship between MEG3 and miR-21-3p; C Experimental strategy for the inhibition of ovarian MEG3 expression by viral injection; D Detection of LncMEG3 by RT‒qPCR in ovarian granulosa cells of PCOS rats; E The expression of miR-21-3p was detected by RT‒qPCR in the ovarian granulosa cells of PCOS rats. A-E, N = 6. Data reflect all measurements and are expressed as the mean ± standard deviation. A t-test was used for statistical analysis between two groups, and one-way ANOVA and Tukey's post hoc test were used for statistical analysis between multiple groups. ***P < 0.0001 compared to the normal group; ^### P < 0.0001 compared to the sh-NC group