Fig. 6

Effect of chemogenetic inhibition of LH-LHb on MS inhibition of cocaine-induced reinstatement. A Experimental schedule for cocaine self-administration. B Schematic of cocaine self-administration procedure in rats. C The number of lever presses during cocaine self-administration and extinction training (n = 6). D–F Effect of chemogenetic inhibition of LH-LHb on MS suppression of cocaine priming-induced reinstatement of drug seeking. The number of total active lever (D), infusion (E) and inactive lever (F) pressings during the reinstatement test session (2-h) following a priming injection of cocaine (15 mg/kg, i.p.) in rats. CNO, CNO administration in the rats given cocaine-primed injection; CNO + MS, CNO administration during MS in the rats given cocaine-primed injection; Veh + MS, aCSF infusion during MS in the rats given cocaine-primed injection. *P < 0.001; ^$P < 0.05, Veh + MS versus Rad MS. White bar in each group represents the number of lever responding before cocaine-primed injection. MS, mechanical stimulation of ulnar nerve; Rad MS, mechanical stimulation of radial nerve. n = 6 per group