Fig. 7From: Cholic and deoxycholic acids induce mitochondrial dysfunction, impaired biogenesis and autophagic flux in skeletal muscle cellsIncreased mitochondrial ROS levels induced by DCA and CA in C2C12 myotubes. C2C12 myoblasts were differentiated for 5 days until forming myotubes. Then, cells were incubated with 120 μM of DCA or 500 μM of CA for 72 h. Further, myotubes were incubated with 10 μM or 5 μM of MitoSOX probe for flow cytometry and fluorescence microscopy analysis, respectively. a Representative flow cytometry dot plot analyses to identify mean fluorescence intensity (MFI) for each condition. b–c Analysis of the MFI normalized by the number of cells (n°cells) in myotubes incubated with DCA (b) or CA (c). Values are expressed as the mean of MFI/n°cells ± SEM (n = 3 independent experiments, *p < 0, 05. t-test). (d) Representative images for the MitoSOX signal. , f) Analysis of the MitoSOX fluorescence normalized by TMRE signal in myotubes incubated with DCA (e) or CA (f). Scale bar: 5 μm. Values are expressed as a fold of induction and correspond to the mean ± SEM (n = 3 independent experiments, *p < 0.05. t-test)Back to article page