Fig. 5

The mitochondrial potential is decreased by DCA and CA in C₂C₁₂ myotubes. (a–b) Myotubes were incubated with 120 μM of DCA or 500 μM of CA for 72 h. Then, cells were incubated with a TMRE probe for 30 min. a Representative images for TMRE signal. Phase contrast (upper panel), TMRE (middle panel), and zoom of TMRE signal (lower panel). Scale bar: 5 μm. b–c Analysis for TMRE fluorescence in myotubes incubated with DCA (b) and CA (c). Three independent experiments were quantified with four fields of view (areas) analyzed in 25–30 myotubes per condition per each separate experiment. Values are expressed as percentages and correspond to the mean ± SEM (n = 3 independent experiments, *p < 0.05. t-test)