Fig. 6

Photomicrograph of Masson’s trichrome or orcein stained sections of surgically removed auricular cartilage: A (100 times) and B (400 times) show Masson’s trichrome stained sections of the control group which reveal a pale bluish differentiated tissue (yellow star) at the defective edges that show immature chondrocytes (red arrow). C (400 times) exhibit dark brown elastic fibers when the tissue stained with orcein. D (400 times) and E (400 times) show faint reaction of the newly formed tissue to Masson’s trichrome stain (red arrow) and orcein stain (yellow arrow), respectively in the ADMSCs. F (400 times) and G (400 times) reveal positive reaction of the newly formed cartilage of the BMMSCs treated group to both Masson’s trichrome stain (red arrow) and orcein stain (yellow arrow), respectively. Faint brown coloration of the tissue surrounding the formed cartilage was noticed (yellow star) indicating the tissue is formed mainly of collagenous fibers. H (400 times) reveal EMSCs treated group with pale blue colored immature cartilage when stained with Masson’s trichrome stain (red arrow). I (400 times) shows the tissue orcein stained faint brown coloration of the newly formed tissue (yellow star) at the defective edges of the original cartilage that exhibit dark brown branching mature elastic fibers (yellow arrow)