Table 2 Characteristics of nine microsatellite loci developed for Eremanthus erythropappus from Minas Gerais State, Brazil
Locus | Primer sequences (5′-3′) | Repeat motif | Size | Tm (°C) | GenBank |
|---|---|---|---|---|---|
ERE02 | F: TCTTGCTTACGCGTGTGACT | (GA)21 | 119 | 53 | MK075833 |
R: TGCATCCACTCCAATCACTT | |||||
ERE03 | F: GAAGGGAGACATCGGAAGAA | (CTT)5; (CTT)9; (CTT)10; (CTT)8 | 232 | 53 | MK075834 |
R: ACGGAACGGAGAAGAAGAAA | |||||
ERE04 | F: CAGTGAGGGGAAGGGAGAAT | (CTT)37 | 398 | 53 | MK075835 |
R: CCTCCACTATAGGGCGGAAT | |||||
ERE07 | F: GCGTGGGACTAACCCATTC | (CTT)9 | 120 | 53 | MK075836 |
R: ACCTGTTGGTGAAAGGATGC | |||||
ERE08 | F: GAGCCTTCCATGGGAGTAGG | (AGC)5 | 238 | 53 | MK075837 |
R: TGGGAGGGAGAAATTGAACA | |||||
ERE09 | F: GCTTACGCGTGGGACTAACT | (CA)3; (GA)8; (GTA)6 | 269 | 53 | MK075838 |
R: GCGTGGACTAGGAAAACGAA | |||||
ERE10 | F: GATCATCGCCATGAAGGAAT | (GAA)3; ATA; (GAA)27 | 244 | 53 | MK075839 |
R: CAGTGAGGGGAAGGGAGAAT | |||||
ERE13 | F: GAGACCCTGGCTGTCTTCAT | (CT)6; (CA)6 | 378 | 53 | MK075840 |
R: GCGTTGAGTTTCGGAGAAGT | |||||
ERE14 | F: CATCGATTTGGAGGCTTCAT | (CT)11; (AT)8; (GT)18 | 207 | 53 | MK075841 |
R: TGCTTACGTGTGCTCTTGCT |