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Fig. 6 | Biological Research

Fig. 6

From: The expression of heterologous MAM-7 in Lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen Vibrio parahaemolyticus in vitro

Fig. 6

Competitive exclusion of L. rhamnosus to protect CaCo-2 cell line from V. parahaemolyticus colonization. The figure shows the results about the role of L. rhamnosus strains in the inhibition of V. parahaemolyticus from colonizing colon carcinoma cell line (CaCo-2) monolayers. The strains used were V. parahaemolyticus RIMD 2210633 (RIMD), L. rhamnosus wild type (L. rhamnosus), L. rhamnosus pCWA-MAM-7 (pCWA-MAM7) and L. rhamnosus pSEC-MAM-7 (pSEC-MAM7). Values are expressed as the adhesion percentage of V. parahaemolyticus after 3 h of L. rhamnosus wild type (L. rhamnosus– RIMD), pCWA-MAM-7 (pCWA-MAM7– RIMD) or pSEC-MAM-7 (pSEC-MAM7– RIMD) strains monolayer colonization. V. parahaemolyticus adhesion, without previous Lactobacillus treatment, is expressed as “Adhesion” bar. The figure shows values expressed as the mean ± standard deviation of three full biological replicates, each time in technical triplicate. *p < 0.001 (Student’s-Test)

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