Fig. 1

Screening of apoptosis inducing plant extracts. a Graph showing efficacy of plant extracts screened for cytotoxicity. HeLa cells were treated with plant extracts (80 μg/ml) and the viable cells were measured with the Cell Titer 96 Proliferation Assay (Promega). b Graph showing the dose depedent inhibton of cell proliferation. Cells were treated either with DMSO or with the indicated concentration of ZALE and the viable cells were measured as above. The data represent the percentage growth compared with DMSO. c Left panel quantification of apoptotic nuclei observed in HeLa cells treated with either DMSO or ZALE with the indicted extract concentration for 48 h. Right panel representative DAPI stained images. Arrows indicate apoptotic fragmented nuclei