Fig. 2

Human recombinant leptin able initiate sprouting of new vessels from existing one. Light microscopic images of CAM treated with 1, 3 and 5 μg of HRL and control with 1X PBS for 72 h (Figures b, d, f, h are the enlarged version of a, c, e, g respectively). Images of CAM treated with 3 and 5 μg of HRL (e, f, g and h) shows the presences of branches and sub branches from the main blood vessels as an indication of new blood vessel formation by sprouting and CAM treated with 1 μg of HRL (c and d) shows the presence of bulging like structures as an indication of beginning of sprouting of blood vessels when compared to control (a and b). Among 3 and 5 μg of HRL 3 μg shows the presences of more branches and sub branches at the treated area. Figure represents the images recorded at 72 h of incubation using light microscope at 20X magnification of bar is 20 μM and are representative of 3 set of experiments. Arrow indicates the presence of branches and sub branches from the main vessel