Figure 3

Western Blot analysis of protein levels of integrin-β1 and β5, Runx 2, OPN and Col I. Integrin-β1 or integrin-β5 siRNA reduced protein level of integrin-β1 or integrin-β5 (A). B was the image of Western Blot analysis of Col I, OPN, Runx 2. The mechanical tensile strain increased protein levels of Runx 2, OPN and Col I (the first and second column of C, D and E), pretreatment of integrin-β1 siRNA inhibited the tensile strain induced the increment of the three proteins levels. Knockdown of integrin-β5 had little effect on these protein levels, and knockdown of both integrin-β1 and integrin-β5 simultaneously also attenuated the elevation, but the inhibitory effect was less than only integrin-β1 knockdown. *P < 0.05, **P < 0.01, between indicated groups.