Figure 4

Colocalization of ADAM10 and ADAM17 in isolated germ and Sertoli cells. Isolated germ and Sertoli cells were double labelled with anti-ADAM10 (green) and anti-ADAM17 (red). ADAM10 and ADAM17 colocalise in pachytene, round spermatids and Sertoli cells.). Mature caudal epididymis sperm showed immunoreactions against ADAM10 and ADAM17 antibodies in the head (arrowhead) and tail. The last column shows the negative controls (without the primary antibody) merged with the bright field picture. Bars in spermatocyte and round spermatids: 5 μ m, sperm: 20 μ m, and Sertoli cell: 50 μ m.