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Figure 3 | Biological Research

Figure 3

From: Assessment of changes in the brca2 and p53 genes in breast invasive ductal carcinoma in northeast Brazil

Figure 3

PCR-RFLP analysis of p53 gene (Arg/Pro genotype). a: p53 codon 72. M: Molecular weight marker (Promega 50 bp). Heterozygous genotype Arg/Pro; Proline genotype showing lack of restriction site (353 pb band); Arginine genotype cleaved by the enzyme resulting in two fragments (214 and 139 bp). b: p53 codon 249. M: Molecular weight marker (Promega 50 bp); Arrow indicates the fragment corresponding to 249Ser genotype (154 pb) not cleaved by HaeIII; Enzyme digestion fragments showing the Arg249 genotype with 92 and 62 bp.

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