Fig. 4

Steamer-like element 1 responds to BrdU treatment. After collection, hemocytes of 20 different animals were seeded in 24 well plate in triplicates and cultured at 10 ºC for 24 h. Then, DMSO or 100mM BrdU was added to each well. Cells were lysed with Trizol at 24–48 h and RNA was extracted to determine the levels of MchSLE1, MchSLE2 and αTubulin transcripts by RT-qPCR. A. Relative expression of MchSLE1 upon BrdU treatment. B. Relative expression of MchSLE2 upon BrdU treatment. In both, the sum of three experiments performed independently is presented and the DMSO treatment was arbitrary set to 1 for each animal. Please note that Y axis are not in the same scale. One way ANOVA was performed, ns = non-significant, *=p < 0.05. Error bars = SEM