Mutations in BRCA1, BRCA2 and other breast and ovarian cancer susceptibility genes in Central and South American populations

Breast cancer (BC) is the most common malignancy among women worldwide. A major advance in the understanding of the genetic etiology of BC was the discovery of BRCA1 and BRCA2 (BRCA1/2) genes, which are considered high-penetrance BC genes. In non-carriers of BRCA1/2 mutations, disease susceptibility may be explained of a small number of mutations in BRCA1/2 and a much higher proportion of mutations in ethnicity-specific moderate- and/or low-penetrance genes. In Central and South American populations, studied have focused on analyzing the distribution and prevalence of BRCA1/2 mutations and other susceptibility genes that are scarce in Latin America as compared to North America, Europe, Australia, and Israel. Thus, the aim of this review is to present the current state of knowledge regarding pathogenic BRCA variants and other BC susceptibility genes. We conducted a comprehensive review of 47 studies from 12 countries in Central and South America published between 2002 and 2017 reporting the prevalence and/or spectrum of mutations and pathogenic variants in BRCA1/2 and other BC susceptibility genes. The studies on BRCA1/2 mutations screened a total of 5956 individuals, and studies on susceptibility genes analyzed a combined sample size of 11,578 individuals. To date, a total of 190 different BRCA1/2 pathogenic mutations in Central and South American populations have been reported in the literature. Pathogenic mutations or variants that increase BC risk have been reported in the following genes or genomic regions: ATM, BARD1, CHECK2, FGFR2, GSTM1, MAP3K1, MTHFR, PALB2, RAD51, TOX3, TP53, XRCC1, and 2q35. Electronic supplementary material The online version of this article (doi:10.1186/s40659-017-0139-2) contains supplementary material, which is available to authorized users.


Background
Breast cancer (BC) is the most common malignancy among women worldwide. Each year, 1.15 million new cases are diagnosed, representing 23% of all cancer diagnoses among women [1,2], and one in eight women will develop BC during their lives [3]. The greatest challenge currently facing clinical researchers, therefore, is identifying prevention strategies that would reduce the morbidity and mortality associated with the disease.
Breast cancer (BC) is a complex disease, with both sporadic and familial presentations, as in most cancers. Inherited genetic risk factors contribute to BC susceptibility in both familial and sporadic BC.

Open Access
Biological Research *Correspondence: ljara@uchile.cl of age of 40-87% for BRCA1 carriers and 27-84% for BRCA2 carriers. The corresponding ovarian cancer risks are 16-68% for BRCA1 carriers and 11-30% for BRCA2 carriers. Disease-causing mutations are distributed throughout the entire coding regions of both genes. Since the identification of BRCA1/2 as the principal genes responsible for inherited BC [5,20], over 3781 distinct DNA sequence variants have been added to the BIC database (http://research.nhgri.nih.gov/bic/). Of these, 3079 are classified as pathogenic, including 1598 truncating mutations (1197 frameshift and 387 nonsense) and 14 splicing alterations. The frequency of BRCA1/2 mutations varies significantly according to geographic region and ethnicity.
There is a consensus that mutations in genes BRCA1/2 and TP53 are responsible for on average 16-20% of the risk for familial BC [6,7]. Genome-wide linkage analyses using large samples of BRCA1/2-negative families have not mapped any other high-penetrance susceptibility loci to date [21]. Therefore, a large part of the genetic component remains unidentified. How can the remaining ~ 80% of familial BC risk be explained? Ford et al. [15] proposed that other susceptibility alleles, called moderate-or lowpenetrance, could be responsible for a significant percentage of BC in BRCA1/2-negative families. Currently, BC risk variants can be classified into three categories of penetrance (high, moderate, and low) that reflect the probability of developing the disease [22]. Therefore, in non-carriers of BRCA1/2 mutations, disease susceptibility may be explained by mutations in other high-, moderate-or low-penetrance genes, interactions between alleles involved in the same pathways, or environmental factors. Sporadic BC is the result of serial stepwise accumulation of acquired and uncorrected mutations in somatic genes that are yet to be identified [23]. Nevertheless, in cases without a family history of BC (sporadic BC), certain combinations of low-penetrance alleles that are associated with a high polygenic risk score (PRS) have been shown to contribute to BC susceptibility [22].
Screening for BRCA1 and BRCA2 mutations provides potentially significant health benefits. Armed with genetic results, physicians may offer risk-reducing options for mutation carriers who have, thus far, not developed cancer, such as prophylactic mastectomy and oophorectomy, prophylactic tamoxifen, or surveillance [24][25][26][27][28].
Research evaluating the distribution and prevalence of BRCA1/2 mutations in Central and South American populations has been quite limited as compared to the number of studies in North America, Europe, Australia and Israel. Moreover, some of the studies performed in Latin America have analyzed hereditary BC, while others have evaluated early-onset BC or cohorts unselected for family history. Furthermore, because Central and South American populations are of mixed ethnic origin, the distributions of recurrent mutations vary by region and country. Published data regarding other BC susceptibility genes is even scarcer than data on BRCA1/2 mutations. Therefore, the aim of this review is to provide a report on the current state of knowledge regarding pathogenic point mutations and large genomic rearrangements (LGRs) in BRCA1 and BRCA2, as well as mutations in other BC susceptibility genes, in Central and South American populations.

Methods
PubMed, EBSCO, and SciELO databases were searched for all studies involving BRCA1 and BRCA2 mutations in Central and South American individuals with breast cancer. Moreover, we searched for pathogenic mutations or variants in other susceptibility genes in the same populations. The search terms included "hereditary breast cancer;" "South America, " "Latin America, " and other terms associated with Central or South American countries; and "BRCA1 and BRCA2″ and "genes and breast cancer risk. " Manuscripts published through February 28, 2017 were considered. Only papers published in English or Spanish were reviewed. Non-human studies, in vitro or in vivo studies, and studies focused on topics other than breast/ovarian cancer were excluded.
The inclusion criteria varied significantly among the selected studies; therefore, we classified the articles into three categories: cohorts that included cases with hereditary BC (cohort A), cases with early-onset (≤ 40 years) BC (cohort B), and cases unselected for family history of BC (cohort C). We classified a cohort as hereditary BC (cohort A) if the inclusion criteria met one or more of the following criteria, as established in the literature: (1) At least two first-degree relatives with BC and/or ovarian cancer diagnosed at any age; (2) at least two first-or second-degree relatives with BC diagnosed before the age of 50 years; (3) at least three first-or second-degree relatives with BC with at least one diagnosed before the age of 40; (4) at least one relative with BC diagnosed before the age of 50 and at least one relative with ovarian cancer diagnosed at any age; (5) at least one male relative with BC diagnosed at any age and at least one female relative diagnosed with BC at any age; (6) at least one relative diagnosed with BC before the age of 30 and one other first-or second-degree relative diagnosed with BC at any age; and (7) at least one relative with bilateral BC and one other first-or second-degree relative with BC. A cohort was classified as early-onset BC (cohort B) if the cohort was made up entirely of BC patients diagnosed at or before 40 years of age. We classified a cohort as unselected for family history (cohort C) if none of the criteria for hereditary BC were applied in the case selection.
Pathogenic mutations are base substitutions, deletions, or duplications that inactivate the BRCA proteins. "Recurrent" refers to mutations present in several cases in at least one cohort.

The scope of BRCA1 and BRCA2 mutations in Central and South American countries
We conducted a literature review of reports on BRCA1 and BRCA2 pathogenic point mutations and LGRs in 12 Central and South American countries (Argentina, Bolivia, Brazil, Chile, Colombia, Costa Rica, Ecuador, Mexico, Paraguay, Peru, Uruguay and Venezuela). Between January 2002 and February 2017, there were 28 published reports on BRCA mutations in these countries. Figure 1 shows that studies were performed in nine countries: Argentina, Brazil, Colombia, Costa Rica, Chile, Mexico, Peru, Uruguay and Venezuela. There were no reports on BRCA mutations in Bolivia, Ecuador or Paraguay. Collectively, the 28 studies screened 5956 individuals and identified 190 different pathogenic mutations (Additional file 1: Table S1; Tables 1, 2).
Additional file 1: Table S1; Tables 1 and 2 show the cohort size, inclusion criteria, and BRCA pathogenic point mutations, LGR(s) and recurrent mutations detected in cohorts A, B and C, respectively. Additional file 1: Table S1 show that in hereditary BC, 118 different BRCA point mutations were detected in 9 countries (68 in BRCA1 and 50 in BRCA2). Recurrent mutations were detected in Argentina, Chile, Brazil, Colombia and Costa Rica. Table 1 shows that in early-onset BC, 21 different BRCA mutations were detected in Brazil and Mexico (13 in BRCA1 and 8 in BRCA2). The c.5266dupC and c.548-?_4185+?del mutations were recurrent in Brazil and Mexico, respectively. Table 2 shows that in cohorts unselected for family history, 51 different BRCA mutations (29 in BRCA1 and 22 in BRCA2) were detected in Brazil, Colombia, Mexico and Peru. Large genomic rearrangements were reported in Argentina, Brazil, Chile, Mexico and Peru.
When the results were analyzed separately for each country, we found that 57 different BRCA mutations were detected in Argentina (32 in BRCA1 and 25 in BRCA2), all in hereditary BC cohorts (n = 40), including 4 recurrent mutations (2 in BRCA1 and 2 in BRCA2). Four LGRs were reported in BRCA1 but none in BRCA2 [29].
In Brazil, 6 studies that collectively screened 1151 individuals with hereditary BC reported 34 different BRCA mutations (24 in BRCA1 and 10 in BRCA2) [30][31][32][33][34][35], including 7 recurrent mutations (5 in BRCA1 and 2 in BRCA2) (Additional file 1: Table S1). In cohort B, a study by Carraro et al. [36] (n = 54) detected another 5 mutations (2 in BRCA1 and 3 in BRCA2), including the recurrent mutation c.5266dupC (3.7%), which was also a recurrent mutation in hereditary BC (Additional file 1: Table S1). Another 3 mutations not seen in cohorts A or B were detected in cohort C (n = 402) (1 in BRCA1 and 2 in BRCA2), including the recurrent mutation c.6405_6409delCTTAA (0.5%) [37]. Therefore, 42 different pathogenic point mutations in BRCA were described in the cohorts A, B and C in Brazil. All patients positive for BRCA mutations had a family history of BC (Additional file 1: Table S1; Tables 1, 2). Four different LGRs Fig. 1 The scope of BRCA1 and BRCA2 mutations in Central and South American countries. In total 12 countries were evaluated. No BRCA mutation studies were found in Bolivia, Paraguay and Ecuador (the latter only with other susceptibility alleles)  Table S1). In Chile, 19 BRCA mutations were reported (9 in BRCA1 and 10 in BRCA2), all in hereditary BC. Of these, 9 were recurrent (4 in BRCA1 and 5 in BRCA2) (Additional file 1: Table S1) [38,39]. Furthermore, 2 LGRs were detected in cohort A [40]. No BRCA mutations were reported in cohorts B or C.
The only study on patients with hereditary BC in Colombia (n = 53) described 6 BRCA mutations (2 in BRCA1 and 4 in BRCA2), 2 of which were recurrent in BRCA1 (c.3331_3334delCAAG and c.5123C>A) and one of which was recurrent in BRCA2 (c.2808_2811delACAA) (Additional file 1: Table S1) [41]. Another 3 studies that collectively screened 1106 patients unselected for family history described another 4 mutations (1 in BRCA1 and 3 in BRCA2) [42][43][44]. Table 3 shows the mutations that were reported in more than one cohort. No LGR studies were performed in Colombia. Therefore, in the Colombian population, 10 different pathogenic point mutations in BRCA were detected, 3 of which were recurrent (Additional file 1: Table S1 and Table 2), and no LGR studies were available.
In Mexico, 17 different BRCA mutations were reported in hereditary BC (10 in BRCA1 and 7 in BRCA2). Three LGRs were also described. The authors did not report recurrent mutations [46,47]. In cohort B, 11 mutations were described (7 in BRCA1 and 4 in BRCA2) [48][49][50]. Of these, 4 mutations in BRCA1 (c.548-?_4185+?del, c.2296-2297delAG, c.3598C>T and c.4327C>T) and 3 in BRCA2 (c.519+5_519+8delGTAA, c.1796-1800delTT-TAT and c.4111C>T) were present in women with earlyonset BC and no family history of the disease [48,50]. In the Mexican patients unselected for family history, 36 different BRCA mutations were described (20 in BRCA1 and 16 in BRCA2) [50,51]. Of these, 12 were also present in cohorts A or B (Table 3). In cohort C, 6 point mutations were recurrent (4 in BRCA1 and 2 in BRCA2), including c.548-?_4185+?del, which was also a recurrent mutation in early-onset BC patients with no family history of the disease. In cohort C, 3 recurrent LGRs were reported. The LGR exon 9-12del had a frequency of 6.9%, making it one of the most frequent BRCA mutations described in the Mexican population.
Three studies were available for Peru. Two studies with cohorts unselected for family history of BC reported 12 different mutations (9 in BRCA1 and 3 in BRCA2). The mutations c68_69delAG, c.1961_1962delA and c.2808_2811delACAA were recurrent, and 2 LGRs were also detected (Table 2) [52,53]. The third publication tested for LGRs in 16 hereditary BC patients but did not test for pathogenic point mutations. The authors detected only one LGR, in BRCA1 (exon 7 amplification) [54].
In Uruguay, only one study described BRCA mutations, in a cohort of 53 patients with heredity BC. Seven mutations were detected (2 in BRCA1 and 5 in BRCA2), and no LGR testing was performed [55].
In Venezuela, only one study reported BRCA mutations, again in patients with hereditary BC (n = 51). The authors described 6 different mutations (3 in BRCA1 and 3 in BRCA2). No recurrent mutations were reported, and no LGR testing was performed [56]. Table 4 shows BRCA1/2 mutations common in more than one Central or South American country, including a total of 21 mutations (14 in BRCA1 and 7 in BRCA2). The most common mutations were found in exons 2, 5, 11, 13, 18 and 20 in BRCA1 and in exons 3 and 11 in BRCA2. Seven mutations were present in 3 or more countries: c.68_69delAG, c.211A>G, c.3331_3334delCAAG and c.5123C>G in BRCA1 and c.145G>T, c.2808_2811delACAA and c.5946delT in BRCA2. The c.68_69delAG mutation, also known as 185delAG (BRCA1 exon 2), was described in Argentina, Brazil, Chile, Mexico and Peru and was reported as a recurrent mutation in Brazil (0.3%), Chile (0.6%) and Peru (2.6%). The mutation c.211A>G (BRCA1 exon 5) was detected in Argentina, Brazil, Mexico and Peru and was reported as a recurrent mutation in hereditary BC in Argentina (1.17%). The c.3331_3334delCAAG was present in BC patients from Brazil, Chile and Colombia and was a recurrent mutation in Chile (0.9%) and Colombia (9.4%). The mutation c.5123C>A (BRCA1 exon 18) was detected in Argentina (cohort A), Brazil (Cohort A), Colombia (cohort A and C) and Mexico (cohort A, B and C) and was a recurrent mutation in Colombia (5.7%) and Mexico (0.5%). In BRCA2, 6 mutations in exon 11 (c.2808_2811delACAA, c.3264dupT, c.4740_4741insTG, c.535dupA, c.5946delT and c.6024dupG) and one in exon 3 (c.145G>T) were detected in more than one country; c.2808_2811delACAA was a recurrent mutation in Argentina (0.64%), Colombia (3.8%) and Peru (0.75%), and c.145G>T was a recurrent mutation in Chile (2.6%).

Other BC susceptibility mutations in Central and South American countries
There is a consensus that BC risk is attributable to susceptibility alleles in many different genes. In patients negative for BRCA1/2 mutations, inherited variations in other genes explain up to 20% of familial BC [8]. However, 51% of breast cancer families do not show mutations in BRCA1/2 or other known susceptibility genes and are therefore classified as BRCAX families. These families may carry a mutation in a moderate-penetrance BC gene yet to be identified. Alternatively, a truly polygenic model may underlie these cases, with susceptibility conferred by the collective actions of several low-penetrance loci [57][58][59][60]. We carried out a literature review of reports on pathogenic mutations or variants in other susceptibility genes in Central and South American countries and found 19 publications between January 2002 and February 2017 in 5 Central or South American countries: Brazil, Chile, Ecuador, Mexico and Peru (Fig. 1). Pathogenic mutations or variants that increase BC risk were reported in the following genes or genomic regions: ATM, BARD1, CHECK2, FGFR2, GSTM1, MAP3K1, MTHFR, PALB2, RAD51, TOX3, TP53, XRCC1 and 2q35.
ATM is frequently implicated in hereditary BC as a low-penetrance susceptibility gene. The ATM kinase has an essential role maintaining genomic integrity, as a key activator of cellular responses to DNA double-strand breaks [61]. In Chile and Mexico, association studies were performed to evaluate the relationship between common ATM variants and familial BC [62,63]. The same variants were studied in both countries: IVS24-9delT and IVS38-8T>C. Both reports concluded that these variants are associated with increased risk of BC (Table 5). In Chile, the authors studied the variant 5557G>A, which was also found to increase BC risk [62].
Germline and somatic mutations in the BARD1 gene are reportedly associated with susceptibility to a subset of breast and ovarian cancers [64]. BARD1 participates in important cellular processes such as DNA repair, RNA processing, transcription, cell cycle regulation and apoptosis [65]. Studies on BARD1 were performed in Chile and Peru (Table 5) [53,66]. Gonzalez-Hormazabal et al. [66] reported that in Chilean women negative for BRCA1/2 mutations, BARD1 Cys557Ser was associated with increased risk of BC. In Peru, one pathogenic mutation (c.334C>T) was reported in one of the triple-negative BC patients studied (0.95%).
CHEK2 is a gene involved in DNA damage and replication checkpoint responses and has been suggested as a BC susceptibility gene. The CHEK2 1100delC variant, which is associated with increased BC susceptibility among familial BC cases not attributable to mutations in BRCA1/2 [67], was studied in Brazilian (n = 120) [31] and Chilean (n = 196) patients with hereditary BC [67]. Only one of the Brazilian patients carried this mutation (0.83%), and it was not present is any of the Chilean cases (n = 196). Therefore, this variant is not a common mutation in these two populations (Table 5). Glutathione S-transferases (GSTs) play an important role in carcinogen detoxification and metabolism of various bioactive compounds [68]. The GST family is composed of six classes of isoenzymes, including GSTM1 [69]. The GSTM1 gene is polymorphic in humans and has three known alleles: GSTM1*A, GSTM1*B and GSTM1O (null), which is the most common variant. The null variant results in undetectable expression of the gene product [70], leading to excessive accumulation of reactive oxygen species and consequently higher susceptibility to carcinogenic events due to DNA damage [71]. Three studies in Mexican and Brazilian populations evaluated the association between the null genotype and BC risk. Two reports concluded that GSTM1O is associated with BC risk in patients from northeastern Mexico [72] and Guadalajara [69]. In Brazil, a study by Possuelo et al. [73] also reported an association between the null GSTM1 genotype and BC risk.
The MTHFR enzyme, encoded by the MTHFR gene, is responsible for catalyzing the irreversible conversion of 5,-0-methylenetetrahydrofolate to 5-methylenetetrahydrofolate. The latter molecule is involved in DNA methylation, an important mechanism in regulation of gene expression. Alterations in DNA methylation due to MTHFR polymorphisms may be associated with the development of cancer [74][75][76]. Association studies on MTHR C677T polymorphisms and BC risk were performed in Brazil [77] and Ecuador [78] (Table 5). In both reports, the authors found a significant association between this SNP and BC risk.
RAD51 is a gene that plays a key role in repairing DNA double-strand breaks through homologous DNA recombination, forming complexes with other proteins involved in DNA repair such as BRCA2 [79,80]. Variants or pathogenic mutations in this gene were studied in Chile [81] and Peru [53]. In Chile, no mutations were detected in the exon or splice-boundaries regions of the RAD51 gene. The same study also evaluated the RAD51 5′UTR variant 135 G>C, which is associated with an increased risk of familial BC in BRCA1/2-negative women and early-onset BC (age < 50 years at diagnosis). In Peru, the pathogenic mutation c.694C>T was detected in triple-negative BC patients (n = 105), with a frequency of 0.95% (Table 5).
Mutations in the TP53 tumor suppressor gene also play a significant role in cancer risk, as impaired p53 function may contribute to the multistep process of carcinogenesis [82]. The p53 protein is important in cell-cycle regulation and maintenance of genome stability. The most notable property of p53 is its action as a transcription factor [83]. We found three articles that studied variations in TP53, all in Brazilian populations [31,84,85]. These articles studied the c.1010G>A (p.R337H) mutation, which occurs at a high frequency in southern and southeastern Brazil [86][87][88][89][90]. Silva et al. [31] reported a frequency of 2.5% for this variant and suggested that all BRCA-negative female BC patients with clinical criteria for hereditary breast-ovarian cancer should be tested for the c.1010G>A variant. Giacomazzi et al. [84] reported that the prevalence of p.R337H was higher in women diagnosed with BC at or before 45 years of age (12.1%) than in those diagnosed at 55 or older (5.1%). An article by Andrade et al. [85] suggested that screening for the germline TP53 p.R337H mutation should be recommended for young females with no family history of cancers associated with Li-Fraumeni syndrome. The three authors agree that inheritance of the c.1010G>A variant may significantly contribute to the high incidence of BC in Brazil.
The XRCC1 gene encodes a protein involved in DNA base excision repair. Therefore, mutations or polymorphisms in this gene may be involved in the genetic etiology of BC. The only study on the association between the XRCC1 gene and BC risk was performed in a Mexican population [91]. Macias-Gomez et al. [91] studied Arg1945Trip and Ag399Gln, reporting a significant association between BC risk and the 399Gln polymorphism but no significant association with the Arg194Trip polymorphism.
Fibroblast Growth Factor Receptor 2 (FGFR2) and mitogen-activated protein kinase-kinase-kinase 1 (MAP3K1) have been proposed as low-penetrance BC susceptibility genes [57]. A study by Jara et al. [92] used a case-control design to evaluate the association of BC with the FGFR2 SNPs rs2981582, rs2420946 and rs121648 and the MAP3K1 SNP rs889312 in BRCA1/2negative Chilean BC cases. All of the SNPs studied were significantly associated with increased BC risk in familial BC and non-familial early-onset BC, in a dose-dependent manner. In Mexico, a study by Murillo-Zamora et al. [93] reported that rs2981582 was associated with BC risk (p = 0.007) ( Table 5).
In the TOX3/LOG643714 (also known as TNRC9) locus, several SNPs associated with BC risk were identified. Among these, rs380362 is the most strongly correlated with disease [57]. The SNPs rs13387042 (2q35) and rs13281615 (8q24), located in non-coding regions, were also associated with BC risk [57,60]. In a Chilean population, Elematore et al. [94] evaluated the association between rs380362 (TOX3), rs13387042 (2q35) and rs13281615 (8q24) and BC risk in 344 BRCA1/2-negative BC cases and 801 controls. Two SNPs, rs380362 and rs13387042, were significantly associated with increased BC risk in familial BC and non-familial early-onset BC. The risk of BC increased in a dose-dependent manner with the number of risk alleles (p-trend < 0.0001 and 0.0091, respectively). Other studies reported an additive effect of the rs380362 and 2q35 rs1387042 alleles on BC risk. There was no association between rs13281615 (8q24) and BC risk ( Table 5). The PALB2 (partner and localizer of BRCA2) protein interacts with BRCA2, stabilizing the intracellular accumulation of the BRCA2 protein at sites of DNA damage [96]. PALB2 is also recruited by BRCA1 in response to DNA damage and serves as a linker between BRCA1 and BRCA2 and is necessary for BRCA2-mediated homologous-recombination repair [97,98]. Thus, BRCA1, BRCA2 and PALB2 are key BC susceptibility genes that work together in the same DNA damage response pathway [99,100]. Leyton et al. [95] studied 100 BRCA1/2-negative Chilean cases with familial BC, identifying 3 PALB2 variants. Using a casecontrol design, the authors evaluated the association of the identified variants with BC risk. Two of the variants, PALB2 c.1676A>G(rs152451A>G) and c.2993C>T (rs45551636C>T), were significantly associated with increased BC risk only in cases with a strong family history of BC (Table 5).

The relationship of BRCA1/2 mutations and other BC susceptibility variants to the demographic composition of Central and South American countries
Genetic factors play an important role in the development of BC. The most widely-accepted model of BC oncogenesis, known as the polygenic model, attributes BC susceptibility to a small number ethnicity-specific mutations in high-penetrance genes (BRCA1, BRCA2 and TP53) and a much larger number of variants in moderate-or low-penetrance genes [7,101], as well as interactions among these genetic variants and exposure to environmental factors [102]. Both BRCA1 and BRCA2 confer susceptibility to breast and ovarian cancer. About 5-7% of all BC diagnosed are associated with germline mutations in BRCA1 and BRCA2 [8,15], and an even larger proportion of familial BC cases are associated with BRCA1 and BRCA2 variations; collectively, germline mutations in the two major susceptibility genes BRCA1 and BRCA2 account for ~ 20% of familial BC cases [8,103]. The spectrum of mutations in BRCA1 and BRCA2 genes and other susceptibility alleles varies considerably by ethnic group and geographic region.
South America has a complex demographic history shaped by multiple migration and admixture events in pre-and post-colonial times [104], including settlement by Native Americans, European colonization and the African slave trade [104]. Moreover, the continental ancestry of the admixed populations in South America is not homogenous. For example, the Argentine population is a mixture of European (0.673), Native American (0.277), West African (0.036) and East Asian (0.014)   González-Rivera et al. [53] components, while the proportions in the Peruvian population are European (0.26), Native American (0.683), West African (0.032) and East Asian (0.025) [104]. Uruguay is unique among South American countries in that it has almost no communities of Native American or African descent [105]. Therefore, South American countries should not be analyzed as a monolithic group without regard for specific regional genetic ancestry, as the ethnic differences between South American populations suggests that medically-relevant genetic variations may differ according to population and region. Mexico and Costa Rica were the only Central American populations with data on BRCA mutations. Central America was included in this review as it was also colonized by Spaniards. The Costa Rica population is a mixture of European (0.61), Native American (0.31) and African (0.06) components, with variations by region [106]. For example, a recent study on the genetic and population substructure in Guanacaste, Costa Rica, which is heavily admixed, reported a mixture of predominantly European (0.425), Native American (0.383) and African (0.152) ancestry, although the authors could not exclude an Asian component (0.04) [107].
The Mexican population also harbors great ethnic diversity [108] as confirmed by numerous studies on the admixture in Mexico. Amerindian ancestry is the largest component (0.51-0.56) in the general population, followed by European (0.40-0.45), while the African component is small (0.02-0.05). When analyzed by region, however, there is significant variation. For example, European is the largest component in the north (at 0.5 in Chihuahua, 0.62 in Sonora and 0.55 in Nueva Leon) [105].
An overview of the literature indicates a marked Amerindian influence in Mexican and Peruvian populations, while European ancestry is more prevalent in Costa Rica, Argentina and Uruguay. The proportions of European, Amerindian and African components are roughly equal in Venezuela. In Colombia and Brazil, there is significant interpopulation variability. The ethnic distribution in Brazil follows a geographical pattern, with the European influence more prevalent in the southeast and south, African in northeast and Amerindian in the north. In Chile, the Amerindian and European components are 0.6 and 0.4, respectively [105].

Genetic testing for breast cancer
Genetic testing for BRCA1 and BRCA2 mutations may provide significant public health benefits for cancer patients and high-risk individuals, who could be offered targeted treatment and prevention strategies [109]. The feasibility of providing widespread genetic screening for BRCA1/2 mutations in Central and South America depends on knowledge of mutations present in these regions, given the varied ethnic composition of the populations. To develop a test that might be useful throughout the region and therefore sufficiently cost-effective, it is first necessary to determine which BRCA1/2 mutations are common in multiple countries. Public insurance coverage for genetic testing is also crucial. Finally, it is important to identify pathogenic mutations or variants in other moderate-or low-penetrance susceptibility genes that increase BC risk, as the use of panel testing is growing more common.

Conclusions
The BRCA1/2 gene mutation spectrum varies widely throughout different Central and South American populations, likely due to the patterns of ethnic diversity in these countries. These complex ethnic patterns are associated with various migration and settlement events. Even populations within a given country are not necessarily homogeneous, and each subgroup may have a distinct ethnic composition and genetic structure. Because the same genetic composition cannot be extrapolated across diverse sub-populations, genetic screening tests for breast cancer in these regions should not be based on a single genetic test with a defined gene variant panel to detect mutational events. This guideline is even more categorical for screening approaches designed to test more than one population in Central and or South American countries.
A significant percentage of high-risk families with hereditary breast cancer are negative for mutations in BRCA1/2 genes. The genetic etiology of BC in these subjects may be attributable to variations in other moderateor low-penetrance susceptibility alleles and/or variations in specific chromosomal regions. Data on variants in these genes and/or chromosomal regions in Central and South American populations are even scarcer than studies involving high-penetrance alleles. Given the importance of these variants in the etiology of hereditary BC, elucidating the distribution of these mutations and variations is crucial for advancing population studies and screening approaches in high-risk families with a hereditary breast cancer profile.
Appropriate inclusion criteria are also of vital importance when conducting these studies, given the considerable variability observed in the reported studies.

Additional file
Additional file 1: Table S1. Cohort characteristics and pathogenic BRCA1 and BRCA2 mutations in hereditary breast cancer in Central and South American populations.