Fig. 1

The pain threshold of rats decreased and the dendritic spines of the spinal dorsal horn were abnormally reconstructed after SNL. A Schematic diagram of the experimental process. B Mechanical withdrawal threshold (MWT). n = 15. ^##p < 0.01 vs. the sham group. C Thermal withdrawal latency (TWL). n = 15. ^##p < 0.01 vs. the sham group. D, E Example of dendritic spines of spinal dorsal horn neurons (the selected site of neurons was I-IV lamina). F Schematic diagram of the classification of thin and mushroom-shaped dendritic spines. G Dendritic spines. Scale bars, 10 μm. H, I Five neurons were randomly selected from the dorsal horn of the spinal cord of each rat, and 20 to 30 dendritic spines were randomly selected from each neuron, and the lengths and head diameters of dendritic spines were measured. n = 4. J Number of spines per 10 μm. n = 4. K Number of thin spines per 10 μm. n = 4. L Number of mushroom spines per 10 μm. n = 4. Columns represent the mean ± SD. ^*p < 0.05, ^**p < 0.01 vs the sham group; ^&p < 0.05, ^&&p < 0.01 vs. the SNL (10) group