Fig. 2

Functional characterization in RDEB-CW fibroblasts. (a-c) Representative bright field images of fibroblast cultures stained with the senescence associated β-galactosidase marker. β-Gal + cells were delimited with a dashed blue line. The quantification of β-Gal + cell population and the measurement of the cell area for β-Gal + and β-Gal- cells are depicted in (b) and (c), respectively. For the quantitative analysis in (b), a total of ~ 4000 cells were used per condition. For the quantitative analysis in (c), a total of 50 cells were used for each condition (150 cells per experimental group). Bar: 100 μm. (d) Representative fluorescence images of fibroblast cultures stained with Lamin B1 (red), and counterstained with DAPI (blue). Bar: 100 μm.  (e) Fluorescence-based proliferation assay (Cell Titer Blue ®) assessed every 24 h during 72 h.  (f) Representative brightfield images of 2D migration assay. Bar: 100 μm. (g) Quantification of migration rate, expressed as percentage of the initial wound area. All data are expressed as mean ± SD. All these experiments were performed with n = 3–4 per condition. Asterisks indicate significant differences by one-way ANOVA with Tukey post-hoc (p < 0.05)