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Fig. 1 | Biological Research

Fig. 1

From: Maintenance of chronicity signatures in fibroblasts isolated from recessive dystrophic epidermolysis bullosa chronic wound dressings under culture conditions

Fig. 1

General phenotypic characterization of RDEB-CW cells. (a) Western blot of protein lysates derived from fibroblast cultures confirms the C7 deficiency in all samples coming from RDEB patients used in this study, with its respective densitometric analysis in (b). (c) Representative IIF images showing molecular markers for fibroblasts (FAP, vimentin) and white blood cells (CD45). Nuclei were co-stained with DAPI. Bar: 100 μm. (d-f) Representative bright field images of NHFs, RDEBs, RDEB-CWs and RDEB-AWs fibroblast cultures used for morphological evaluation. Black arrows indicate cell protrusions. The morphological parameters analyzed were cell area (e) and the mean protrusions per cell (f). For the quantitative analysis in (e-f), a total of 75 cells were used for each condition (225 cells per experimental group). Bar: 100 μm. All data are expressed as mean ± SD. All these experiments were performed with n = 3–4 per condition. Asterisks indicate significant differences by one-way ANOVA with Tukey post-hoc (p < 0.05)

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