Fig. 7

Purity of monomeric RBD during a stress stability at 37 °C measured by GF-HPLC and SDS PAGE. A mRBD chromatograms by GF-HPLC during storage at 37 °C. B XY graph of the percentages of the species calculated from the area under the curve of the peaks corresponding to the mRBD. Lots of the mRBD are shown: Point 1: mRBD Lot 3 time 0, Point 2: mRBD Lot 3 time 7 days, Point 3: mRBD Lot 3 time 15 days, Point 4: mRBD Lot 3 time 22 days and Point 5: mRBD Lot 3 time 30 days. Percentage of the mRBD are shown: monomer (blue), dimer (pink), aggregates (red) and fragmentation (green). C Electrophoretic profile by SDS-PAGE of the mRBD at different concentrations of stress (Point 1: MWM, Molecular Weight size Marker, Point 2: mRBD Lot 3 time 0, Point 3: mRBD Lot 3 time 7 days, Point 4: mRBD Lot 3 time 15 days, Point 5: mRBD Lot 3 time 22 days and Point 6: mRBD Lot 3 time 30 days)