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Fig. 6 | Biological Research

Fig. 6

From: Conformational characterization of the mammalian-expressed SARS-CoV-2 recombinant receptor binding domain, a COVID-19 vaccine

Fig. 6

Stress stability at 37 °C of mRBD and dRBD measured by FS. A Lots of the mRBD are shown: mRBD Lot 3 time 0 (black), mRBD Lot 3 time 7 days (red), mRBD Lot 3 time 15 days (blue), mRBD Lot 3 time 22 days (pink) and mRBD Lot 3 time 30 days (green). B Lots of the mRBD are shown: mRBD Lot 3 time 0 (black), mRBD Lot 3 time 7 days (red), mRBD Lot 3 time 15 days (blue), mRBD Lot 3 time 22 days (pink) and mRBD Lot 3 time 30 days (green). FS spectra, where each spectrum represents the fluorescence emission intensity (a.u.) for each wavelength (nm) and is the product of a single scan in the 307–410 nm range. Selective Trp excitation was performed at 295 nm, with 5 nm slits for excitation and emission, respectively. Protein concentration 4.13 µmol/l of mRBD and 2.07 µmol/l of dRBD. Spectra were acquired using a Jasco J-1500 spectropolarimeter (Jasco Corporation, Japan), in quartz cuvettes with a 1 cm light path, at 25 °C. C, D Bar graph of the Trp λmax for the mRBD and dRBD. Trp λmax of the RBD are shown: mRBD (blue) and dRBD (red)

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