Fig. 5

Nedd4-1 ablation results in decreased expansion and differentiation of myogenic progenitors in vivo. A SC-N4wt and SC-N4KO TA muscles were obtained at 5, 7, and 15 days post-injury (dpi), cryosectioned, and analyzed by IF for PAX7 expression (red; arrowheads). Nuclei were stained with DAPI (blue). Scale bar = 30 μm. B Number of myogenic progenitors (PAX7 +) per 100 nuclei from cryosections obtained as in A; n = 3; **P-value < 0.01. C Number of myogenic progenitors (PAX7+) per section area (mm²) from cryosections obtained as in A; n = 3; **P-value < 0.01. D SC-N4wt and SC-N4KO TA muscles obtained as in A were cryosectioned and analyzed by IF for MYOGENIN (MGN) expression (magenta, arrowheads). Nuclei were stained with DAPI (blue). Scale bar = 20 μm. E Number of myogenic progenitors (MGN+) per 100 nuclei from cryosections obtained as in D; n = 3, *P-value < 0.05. F Number of myogenic progenitors (MGN+) per section area (mm²) from cryosections obtained as in D; n = 3, **P-value < 0.01