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Fig. 4 | Biological Research

Fig. 4

From: NEDD4-1 deficiency impairs satellite cell function during skeletal muscle regeneration

Fig. 4

Effect of Nedd4-1 deletion in SCs during late muscle regeneration. A Pax7CreERT2/+; Nedd4-1f/f mice were injected with tamoxifen (TMX) or vehicle (Veh) for five days. 48 h after the final dose, TA muscles were injured by BaCl2 intramuscular injection (see materials and methods). Injured and contra-lateral uninjured TAs were isolated at 30 dpi for further analysis. B Comparison of freshly isolated contra-lateral (uninjured) and injured (30 dpi) TA muscle treated as in A with vehicle (SC-N4wt) or tamoxifen (SC-N4KO) prior to muscle injury. C H&E staining of cryosections from TA muscles obtained as in A at × 4 (left panels) and × 40 (right panels) magnification. Scale bars = 500 μm and 40 μm, respectively. D Differences in average whole TA cross-sectional area (CSA) between contra-lateral (uninj.) and injured (30 dpi) obtained from SC-N4wt and SC-N4KO animals; n = 3, *P < 0.05. E Quantification shows individual fiber CSA for regenerating (centrally located nuclei) myofibers. Uninjured contralateral myofiber CSA are shown as internal control. mean values are indicated for each condition. n = 3, ***P < 0.001

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