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Fig. 2 | Biological Research

Fig. 2

From: NEDD4-1 deficiency impairs satellite cell function during skeletal muscle regeneration

Fig. 2

Nedd4-1 kinetics expression in activated and differentiated SCs. A Skeletal muscle fibers or SCs were isolated from wild-type c57/bl6 adult mice and maintained in proliferation (PM) or differentiation (DM) culture conditions. At the indicated time points, Nedd4-1 expression was evaluated by IF, qPCR, or Western blot (WB). B Isolated skeletal muscle fibers were maintained in proliferation conditions, and NEDD4-1 expression in associated SCs was analyzed by IF (green) at the indicated time points. PAX7 expression was used as SCs marker (red). Nuclei were marked by DAPI staining (blue). Scale bar = 10 μm. C Isolated SCs were maintained in PM for 2 days (2d) or DM for 7 days (7d), and Nedd4-1 mRNA relative levels were measured by qPCR. Pax7, MyoD, and Myogenin mRNA levels were measured to monitor myogenic progression. Freshly isolated (Fi) SCs were used to determine mRNA basal levels. 18S RNA was used as housekeeping for normalization. Results were expressed as the average RQ ± SD; n = 3. *P-value < 0.05; **P-value < 0.01, ***, P-value < 0.001. D Western blot shows the expression of NEDD4-1 in SCs cultures maintained in PM and DM for 2 and 7 days, respectively. For quantitative analysis, GAPDH expression was used for normalization in arbitrary units (A.U); n = 3, **P value < 0.01

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