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Fig. 7 | Biological Research

Fig. 7

From: In situ isolation of nuclei or nuclear proteins from adherent cells: a simple, effective method with less cytoplasmic contamination

Fig. 7

Effects of treatment time of 0.1% or 1% triton on the contents of intranuclear proteins (Hif-1α and pP65) detected by western blotting. a Representative western blot data of two intranuclear proteins (i.e., Hif-1α and pP65) of the HUVEC cells, which were pre-activated by 1 μg/mL lipopolysaccharide (LPS), treated by 0.1% or 1% Triton X-100 for various time periods (0, 10, 30, 60, and 120 min, respectively). b Quantitative analysis of the western blot data from three independent experiments (mean ± SEM). **p < 0.01; *** and p < 0.001; **** compared with the control (i.e., the 0 min group without Triton treatment in which group the total proteins of Hif-1α/pP65, instead of only intranuclear proteins, were harvested for western blotting). Hif-1α: the α subunit of hypoxia inducible factor-1; pP65: phosphorylated P65

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