Fig. 6

Effects of triton treatment at different concentrations on extra- and intra-nuclear proteins detected by western blotting. a Representative western blot data of different extra-/intra-nuclear proteins (LDLR, GAPDH, β-actin, P65, pP65, Hif-1α, Lamin A/C, and H3, respectively at different cellular locations as indicated in the table) of the HUVEC cells treated by Triton X-100 at different concentrations (0, 0.01%, 0.05%, 0.1%, 0.5%, 1%, 2%, and 5%, respectively) for 10 min. b Quantitative analysis of the western blot data from three independent experiments (mean ± SEM). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared with the control (the 0% group). The cells for pP65/Hif-1α detection were pre-activated by 1 μg/mL LPS. LDLR: Low-density lipoprotein receptor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; P65: A subunit of transcription factor NF-κB; pP65: phosphorylated P65; Hif-1α: The α subunit of hypoxia inducible factor-1; H3: Histone H3