Fig. 2

Morphological validation of the in situ isolation of nuclei from adherent cells. a–d In situ confocal microscopic observation of HUVECs treated with 0.01%, 0.1%, and 1% Triton X-100, respectively for 10 min without subsequent PBS washes and with 1% Triton X-100 for 5 min with subsequent PBS washes. Upper panels: before treatment; bottom panels: after treatment. e AFM imaging of HUVECs treated without (left) and with (right) 0.1% Triton X-100 for 10 min. f The height profiles of the cross sections along the lines in AFM images. Upper and bottom panels are corresponding to the left and right panels, respectively in the AFM images. g SEM imaging of HUVECs without treatment. The white arrow indicates the filamentous cytoskeleton in the cytoplasm. h SEM imaging of HUVECs after 0.1% Triton X-100 treatment for 10 min. The white arrowheads indicate the nucleoli inside the nucleus. i, j In situ confocal microscopic observation of HepG-2 cells treated with 0.01% (i) and 0.1% (j) Triton X-100, respectively for 10 min. Left panels: before treatment; right panels: after treatment. Scale bars: (a–e, i, j) 20 μm; (g and h) left panel: 50 μm; right panel: 10 μm