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Fig. 6 | Biological Research

Fig. 6

From: Propofol inhibits myocardial injury induced by microvesicles derived from hypoxia-reoxygenated endothelial cells via lncCCT4-2/CCT4 signaling

Fig. 6

Overexpression of CCT4 abolishes the exacerbating effect of (HR + P)-EMVs silencing lncCCT4-2 on cardiomyocyte injury. Overexpression of CCT4 in AC16 cells and detection of the effect of sh-lncCCT4-2-(HR + P)-EMVs on HR-injured AC16 cells: (A) CCK-8 assay for AC16 cell viability. (B) LDH assay for AC16 cell supernatant lactate dehydrogenase activity. (C) Flow cytometry detection of reactive oxygen species (ROS) levels in AC16 cells after DCFH-DA staining. (D) Quantitative analysis of ROS. (E) Flow cytometry detection of apoptotic rate in AC16 cells after Annexin V-FITC/PI double staining. (F) Quantitative analysis of apoptotic cells. (G-H) Western blotting analysis of Bax, Bcl-2, cleaved caspase-3 in AC16 cells, and the relative gray scales calculated by ImageJ software. These data were representative results (n = 3) of three repetitions. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant. Data are expressed as mean ± SEM.

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Biological Research

ISSN: 0717-6287

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