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Fig. 1 | Biological Research

Fig. 1

From: Propofol inhibits myocardial injury induced by microvesicles derived from hypoxia-reoxygenated endothelial cells via lncCCT4-2/CCT4 signaling

Fig. 1

Characterization and internalization of endothelial microvesicles. (A) Representative TEM image of HR-EMVs, (HR + D)-EMVs and (HR + P)-EMVs. Scale bars, 200 nm. TEM, transmission electron microscopy. (B-C) Nanoparticle tracking analysis (NTA) plots show the size / concentration distribution of HR-EMVs, (HR + D)-EMVs and (HR + P)-EMVs. CD105+ +CD144+ Q-dots EMVs under fluorescence / nonfluorescence modes. Red curve, CD105+ +CD144+ Q-dots EMVs were measured under light scatter (nonfluorescence) mode. Green curve, CD105+ +CD144+ Q-dots EMVs were measured under fluorescence mode. CD105, specific for endothelial cells. CD144, vascular endothelial antigen. (D) Immunofluorescent staining showing the uptake of EMVs by AC16 cells after coculture with PKH26-labelled EMVs for 24 h. Scale bars, 100 μm. HR, hypoxia/reoxygenation; EMVs, endothelial microvesicles

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Biological Research

ISSN: 0717-6287

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