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Fig. 7 | Biological Research

Fig. 7

From: Spleen regeneration after subcutaneous heterotopic autotransplantation in a mouse model

Fig. 7

Experiments on the role of stromal component of the spleen in the engraftment. (A) Structural dynamics of a non-repopulated decellularized scaffold upon subcutaneous transplantation; hematoxylin and eosin (H&E), objective lens 20, scale bar 100 μm. (B) Immunohistochemical visualization of CD68 + cells (PE fluorescence) in a non-repopulated decellularized scaffold upon subcutaneous transplantation; cell nuclei counterstained with DAPI, objective lens 20, scale bar 100 μm. (C) Structural dynamics of a decellularized scaffold repopulated with stromal by coculturing upon subcutaneous transplantation; hematoxylin and eosin (H&E), objective lens 20, scale bar 50 μm. (D) Immunohistochemical visualization CD68 + cells (FITC fluorescence) in a decellularized scaffold repopulated with stromal cells by coculturing upon subcutaneous transplantation; cell nuclei counterstained with DAPI, objective lens 20, scale bar 100 μm. For each term of the study, the experimental group consisted of 5–6 animals

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