Fig. 3
From: Spleen regeneration after subcutaneous heterotopic autotransplantation in a mouse model
Reconstitution of monocyte-macrophage system in the regenerating splenic grafts. (A) Immunohistochemical visualization of CD115 (PE fluorescence) and CD68 (FITC fluorescence), objective lens 40, scale bar 50 μm. The incision on day 7 was taken from the marginal zone of the graft in which cells with a dark nucleus first appeared; on day 30, the incision demonstrates the spread of CD115 + cells throughout the graft. Reconstitution dynamics for CD115 + and CD68 + cells. The data are presented as means ± standard deviations, * p < 0.05 vs. intact spleen. (B) Representative F4/80-SSC scatter plots. Reconstitution dynamics for F4/80 + macrophages. For each term of the study, the experimental group consisted of 5–6 animals. The control group included intact animals (n = 5). The data are presented as means ± standard deviations, * p < 0.05 vs. intact spleen